A Rapid Microassay to Evaluate Enzymatic Hydrolysis of Lignocellulosic Substrates (Record no. 45164)

MARC details
000 -LEADER
fixed length control field 02146nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140641.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10933
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245 10 - TITLE STATEMENT
Title A Rapid Microassay to Evaluate Enzymatic Hydrolysis of Lignocellulosic Substrates
490 0# - SERIES STATEMENT
Volume/sequential designation Biotechnology and BioEngineering, 93(5), p.880-886, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Current attempts to produce ethanol from lignocellulosic biomass are focused on the optimization of pretreatment to reduce substrate recalcitrance and the improvement of enzymes for hydrolysis of the cellulose and hemicellulose components to produce fermentable sugars. Research aimed at optimizing both aspects of the bioconversion process involves assessment of the effects of multiple variables on enzyme efficiency, resulting in large factorial experiments with intensive assay requirements. A rapid assay for lignocellulose hydrolysis has been developed to address this need. Pretreated lignocellulose is formed into handsheets, which are then used to prepare small disks that are easily dispensed into microtiter plates. The hydrolysis of cellulose to glucose is estimated using an enzymecoupled spectrophotometric assay. Using disks prepared from ethanol organosolv pretreated yellow poplar, it is shown that the assay generates data comparable with those produced by hydrolysis of pretreated yellow poplar pulp in Erlenmeyer flasks, followed by HPLC analysis of glucose. The assay shows considerable time and cost benefits over the standard assay protocol and is applicable to a broad range of lignocellulosic substrates.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOMASS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOCONVERSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIGNOCELLULOSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HEMICELLULOSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELLULOSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELLULASE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Berlin, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Maximenko, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bura, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kang, K.Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gilkes, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Saddler, J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/12-heu8HFDLS9ibIQ4XAPRiWiR4Gydgeo/view?usp=drivesdk">https://drive.google.com/file/d/12-heu8HFDLS9ibIQ4XAPRiWiR4Gydgeo/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10933 25.06.2025 25.06.2025 Documentos solicitados