A simplified, competitive RT-PCR method for measuring rat IFN- y mRNA expression (Record no. 45218)
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| 000 -LEADER | |
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| fixed length control field | 02271nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140642.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10990 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A simplified, competitive RT-PCR method for measuring rat IFN- y mRNA expression |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Immunological Methods, 195, p.139-148, 1996 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We describe an adaptation of competitive RT-PCR to quantitate rat IFN--y mRNA expression. An IFN-y DNA mimic that shared the same primers and had an identical sequence to the target mRNA except for deletion of 66 nucleotides, was created by a simple PCR amplification from target cDNA. To reduce variations of initial RNA concentrations, p-actin cDNAs from each target RNA sample were normalized using the densitometric data. A known amount of pretitrated DNA competitor was then used to analyze the relative levels of target cDNA in different samples by PCR co-amplification. The amplification efficiency for both target and competitor remained constant throughout the PCR reaction, and the ratio of target to competitor PCR product remained proportional to the initial ratio of target to competitor. Relative mRNA levels among samples determined by this method were comparable to levels determined by northern blot analysis. They were also comparable to levels of IFN-7 protein estimated by ELISA. We conclude that this method can be used to estimate the relative abundance of the target mRNA. This method is adaptable to quantitation of other cytokines and is particularly valuable if there are numerous samples, or if the amount of initial mRNA is limited. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HOUSEKEEPING GENE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NORTHERN BLOT ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ELISA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA MIMIC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IFN-Y MRNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | (RAT) |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sun, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wells, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Goldmuntz, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Silver, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Remmers, E.F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wilder, R.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Caspi, R.R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/10lblbSmOEiwKrMRqQ7UzgSTHIr3w3_mc/view?usp=drivesdk">https://drive.google.com/file/d/10lblbSmOEiwKrMRqQ7UzgSTHIr3w3_mc/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10990 | 25.06.2025 | 25.06.2025 | Documentos solicitados |