A proteomic evaluation of Pyrenophora tritici-repentis, causal agent of tan spot of wheat, reveals major differences between virulent and avirulent isolates (Record no. 45266)

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fixed length control field 02218nam a2200265Ia 4500
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control field MX-MdCICY
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control field 20250625140642.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11039
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245 10 - TITLE STATEMENT
Title A proteomic evaluation of Pyrenophora tritici-repentis, causal agent of tan spot of wheat, reveals major differences between virulent and avirulent isolates
490 0# - SERIES STATEMENT
Volume/sequential designation Proteomics, 9(5), p.1177-1196, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Pyrenophora tritici-repentis causes tan spot, an important foliar disease of wheat. The fungus produces multiple host-specific toxins, including Ptr ToxB, a chlorosis-inducing protein encoded by the ToxB gene. A homolog of ToxB is also found in avirulent isolates of the fungus. In order to improve understanding of the role of this homolog and evaluate the general pathogenic ability of P. tritici-repentis, we compared the proteomes of avirulent race 4 and virulent race 5 isolates of the pathogen. Western blotting analysis revealed the presence of Ptr ToxB in spore germination and culture fluids of race 5 but not race 4. A comprehensive proteome-level comparison by 2-DE indicated 133 differentially abundant proteins in the secretome (29 proteins)and mycelium (104 proteins)of races 4 and 5, of which 63 were identified by MS/MS. A number of the proteins found to be up-regulated in race 5 have been implicated in microbial virulence in other pathosystems, and included the secreted enzymes a-mannosidase and exo-b-1,3-glucanase, heat-shock and BiP proteins, and various metabolic enzymes. These proteome-level differences suggest a reduced general pathogenic ability in race 4 of P. tritici-repentis, irrespective of toxin production. Such differences may reflect an adaptation to a saprophytic habit.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PATHOGENICITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PTR TOXB
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PYRENOPHORA TRITICI-REPENTIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TAN SPOT OF WHEAT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VIRULENCE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cao, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kim, Y.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kav, N.N.V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Strelkov, S.E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/156eqztelhQMVVlUGXIiDD0nvHsFA6-5R/view?usp=drivesdk">https://drive.google.com/file/d/156eqztelhQMVVlUGXIiDD0nvHsFA6-5R/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11039 25.06.2025 25.06.2025 Documentos solicitados