Spectrophotometric determination of phosphatidic acid via iron(III)complexation for assaying phospholipase D activity (Record no. 45494)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01854nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140647.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-11269 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Spectrophotometric determination of phosphatidic acid via iron(III)complexation for assaying phospholipase D activity |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytical BioChemistry, 392, p.169-173, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The ability of negatively charged phosphatidates to form complexes with Fe3+ ions was used to design a simple spectrophotometric assay for the quantitative determination of phosphatidic acid (PA). In the reaction with the purple iron(III)-salicylate, PA extracts Fe3+ ions and decreases the absorbance at 490 nm. Lower competition with salicylate for Fe3+ ions was observed with single negatively charged phosphatidates such as phosphatidylglycerol (PG), whereas neutral phosphatidates such as phosphatidylcholine (PC)and phosphatidylethanolamine (PE)showed no influence on the absorbance of the iron(III)complex. The detection limit of the method on a microplate scale was 10 lM PA. Based on these results, an assay for determining the activity of phospholipase D (PLD)toward natural phospholipids such as PC, PE, and PG was developed. In contrast to other spectroscopic PLD assays, this method is able to determine PLD activity toward different lipids or even lipid mixtures. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HYDROLYSIS OF GLYCEROPHOSPHOLIPIDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IRON(III)-SALICYLATE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHOSPHATIDIC ACID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHOSPHOLIPASE D ACTIVITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SPECTROPHOTOMETRIC ASSAY |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dippe, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ulbrich-Hofmann, R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1JsDYF1yudaZoLgtuao0gx67H7iOVbuk6/view?usp=drivesdk">https://drive.google.com/file/d/1JsDYF1yudaZoLgtuao0gx67H7iOVbuk6/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-11269 | 25.06.2025 | 25.06.2025 | Documentos solicitados |