A novel method for induction of plant regeneration via somatic embryogenesis (Record no. 45590)

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fixed length control field 02042nam a2200265Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140649.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11371
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245 10 - TITLE STATEMENT
Title A novel method for induction of plant regeneration via somatic embryogenesis
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science, 177(1), p.43-48, 2009
520 3# - SUMMARY, ETC.
Summary, etc. To induce plant regeneration via somatic embryogenesis, a binary expression vector system was constructed containing a heat shock-inducible FRT/FLP system. In this system, the BABY BOOM (BBM)gene, which shows similarity to the AP2/ERF family, was under the control of cauliflower mosaic virus 35S (CaMV 35S)promoter and the gene coding for FLP site-specific recombinase was under the control of a heat shock-inducible promoter HSP18.2 from Arabidopsis thaliana. The promoters and genes were flanked by two FRT recombination sites in direct orientation. BBM overexpression induced formation of somatic embryos from calli of Chinese white poplar (Populus tomentosa Carr.). Six somatic embryos germinated to form transgenic plants with phenotypic alterations. The stem cuttings of the transgenic plants were treated with heat shock and they developed into phenotypically normal plants. Southern blot analysis further confirmed that the BBM gene was excised from the genomic DNA of the transgenic plants with normal phenotype. The system reported here can be used to obtain transgenic plants without the use of antibiotic or herbicide resistance genes and to provide a novel strategy for plant regeneration via somatic embryogenesis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
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Topical term or geographic name entry element REGENERATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BABY BOOM (BBM)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FLP
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HEAT SHOCK PROMOTER
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deng, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Luo, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yang, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Eh_SpaMHChMCPBY-LTDE4hCVYVGBLbI8/view?usp=drivesdk">https://drive.google.com/file/d/1Eh_SpaMHChMCPBY-LTDE4hCVYVGBLbI8/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11371 25.06.2025 25.06.2025 Documentos solicitados