A simple test using restricted PCR-amplifled mitochondrial DNA to study the genetic structure of Apis mellifera L. (Record no. 45628)

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control field MX-MdCICY
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control field 20250625140649.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11409
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245 10 - TITLE STATEMENT
Title A simple test using restricted PCR-amplifled mitochondrial DNA to study the genetic structure of Apis mellifera L.
490 0# - SERIES STATEMENT
Volume/sequential designation Celular and Molecular Life Science, 49(1), p.1016-1021, 1993
520 3# - SUMMARY, ETC.
Summary, etc. The COI-COII intergenic region of Apis mellifera mitochondrial DNA contains an important length polymorphism based on a variable number of copies of a 192 196 bp sequence (Q)and the complete or partial deletion of 67 bp sequence (Po). This length variability has been combined with a restriction site polymorphism to produce a rapid and simple test for the characterization of mtDNA haplotypes. This test includes the amplification by the polymerase chain reaction of the COI-COII region followed by a DraI restriction of the amplified fragment. In a survey of 302 colonies belonging to 12 subspecies, 21 different haplotypes have been found which have been unambiguously allocated to one of the 3 mtDNA lineages of the species. Although all colonies of lineage C exhibit the same pattern (C1), each one of lineages A and M presents up to 10 different haplotypes, opening the way to studies on the genetic structure and the evolution of a large fraction of the species. This test also differentiates southern Spanish and South African colonies, which can be of great interest for the Africanized bee problem.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element APIS MELLIFERA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HONEYBEE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MITOCHONDRIAL DNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RESTRICTION FRAGMENT LENGTH POLYMORPHISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DRAI
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYMERASE CHAIN REACTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COI-COII INTERGENIC REGION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Garnery, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Solignac, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Celebrano, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cornuet, J.M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1VTafyzo6gOareoxaVENyvSNQSoRwFk5u/view?usp=drivesdk">https://drive.google.com/file/d/1VTafyzo6gOareoxaVENyvSNQSoRwFk5u/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11409 25.06.2025 25.06.2025 Documentos solicitados