Effects of Culture Conditions on Cell Division and Composition of Regenerated Cell Walls in Vinca rosea Protoplasts (Record no. 45653)

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control field MX-MdCICY
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control field 20250625140650.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11434
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245 10 - TITLE STATEMENT
Title Effects of Culture Conditions on Cell Division and Composition of Regenerated Cell Walls in Vinca rosea Protoplasts
490 0# - SERIES STATEMENT
Volume/sequential designation Plant & Cell Physiology, 23, p.249-255, 1982
520 3# - SUMMARY, ETC.
Summary, etc. Protoplasts prepared from suspension-cultured Vinca rosea cells were grown in a liquid medium, and the effects of osmolarity and growth regulators on cell division and on the composition of regenerated cell walls were investigated. The concentration of mannitol optimal for cell division was 0.3-0.4 M. The presence of 2,4-D was essential for cell division, and 6-benzylaminopurine (BAP)enhanced cell division at concentrations of 0.03-0.1 ppm. However, the composition of regenerated cell walls was abnormal under suspension culture; the predominant sugar was glucose, indicating that the regenerated cell walls consisted mostly of glucans, and that the other cell-wall components were released into the medium. Mannitol, 2,4-D, and BAP at various concentrations did not significantly affect the sugar composition of the regenerated cell walls. Compared with liquid culture, cell division was stimulated when protoplasts were cultured on agar, and their regenerated cell walls had a composition similar to that of the original culture. The importance of the physical environment for the deposition of polysaccharide components in cell walls and the interrelationship between cell division and the regeneration of cell walls by protoplasts are discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL DIVISION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL-WALL REGENERATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYSACCHARIDES (CELL WALL)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTOPLAST CULTURE (VINCA)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VINCA ROSEA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Takeuchi, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Komamine, A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1UbhcsmFUmF5cBiAVXklDPq6E7InOJ_-w/view?usp=drivesdk">https://drive.google.com/file/d/1UbhcsmFUmF5cBiAVXklDPq6E7InOJ_-w/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11434 25.06.2025 25.06.2025 Documentos solicitados