Extracellular proteomes of Arabidopsis thaliana and Brassica napus roots: analysis and comparison by MudPIT and LC-MS/MS (Record no. 45749)
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| fixed length control field | 02620nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140652.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-11533 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Extracellular proteomes of Arabidopsis thaliana and Brassica napus roots: analysis and comparison by MudPIT and LC-MS/MS |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Soil, 286, p.357-376, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | An important principle of the functional organization of plant cells is the targeting of proteins to specific subcellular locations. The physical location of proteins within the apoplasm/ rhizosphere at the root-soil interface positions them to play a strategic role in plant response to biotic and abiotic stress. We previously demonstrated that roots of Triticum aestivum and Brassica napus exude a large suite of proteins to the apoplasm/rhizosphere [Basu et al. (1994)Plant Physiol 106:151-158; Basu et al. (1999)Physiol Plant 106:53-61]. This study is a first step to identify low abundance extracytosolic proteins from Arabidopsis thaliana and Brassica napus roots using recent advances in the field of proteomics. A total of 16 extracytosolic proteins were identified from B. napus using two-dimensional gel electrophoresis, tandem mass spectrometry (LC-MS/MS)and de novo sequencing. Another high-throughput proteomics approach, Multidimensional Protein Identification Technology (Mud PIT)was used to identify 52 extracytosolic proteins from A. thaliana. Signal peptide cleavage sites, the presence/absence of transmembrane domains and GPI modification were determined for these proteins. Functional classification grouped the extracellular proteins into different families including glycoside hydrolases, trypsin/ protease inhibitors, plastocyanin-like domains, copper-zinc superoxide dismutases, gamma-thioinins, thaumatins, ubiquitins, protease inhibitor/ seed storage/lipid transfer proteins, transcription factors, class III peroxidase, and plant basic secretory proteins (BSP). We have also developed an on-line, Extracytosolic Plant Proteins Database (EPPdb, http://eppdb.biology.ualberta. ca)to provide information about these extracytosolic proteins. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Basu, U. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Francis, J.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Whittal, R.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Stephens, J.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zaiane, O.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Goebel, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Goebel, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Taylor, G.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1wSwsfGRhvVvyzA9Z7EDzZ08QdAf1JW9u/view?usp=drivesdk">https://drive.google.com/file/d/1wSwsfGRhvVvyzA9Z7EDzZ08QdAf1JW9u/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-11533 | 25.06.2025 | 25.06.2025 | Documentos solicitados |