Monitoring dark hydrogen fermentation performance of indigenous Clostridium butyricum by hydrogenase gene expression using RT-PCR and qPCR (Record no. 45756)

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fixed length control field 01926nam a2200277Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140652.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11540
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245 10 - TITLE STATEMENT
Title Monitoring dark hydrogen fermentation performance of indigenous Clostridium butyricum by hydrogenase gene expression using RT-PCR and qPCR
490 0# - SERIES STATEMENT
Volume/sequential designation International Journal of Hydrogen Energy, 33(18), p.4730-4738, 2008
520 3# - SUMMARY, ETC.
Summary, etc. Hydrogenase is the key enzyme responsible for H2 production in dark fermentation. Therefore, the expression of hydrogenase gene may be a good indicator for the performance of a dark H2 fermentation culture. In this study, we investigated the correlation between expression of the functional gene (hydA encoding for hydrogenase in Clostridium butyricum)and bioH2 production activity during batch growth of an indigenous H2-producing isolate C. butyricum CGS5 using sucrose as the sole carbon source. The copy number of hydA mRNA was determined by using reverse transcription PCR (RT-PCR)and quantitative PCR (qPCR). The results show that the specific hydrogen production rate of C. butyricum CGS5 was essentially linearly proportional to the level of hydA expression (represented by the copy umber of hydA cDNA), whereas the profiles of microbial growth and volumetric H2 production rate followed a similar trend to that of the hydA DNA copies.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DARK H2 FERMENTATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CLOSTRIDIUM BUTYRICUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYDA GENE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REVERSE TRANSCRIPTION PCR (RT-PCR)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element QUANTITATIVE PCR (QPCR
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, M.Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tsai, Y.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Olson, B.H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chang, J.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1jCNtFeJxc7ctGiib5KvJ1-5f6Yxc9HgH/view?usp=drivesdk">https://drive.google.com/file/d/1jCNtFeJxc7ctGiib5KvJ1-5f6Yxc9HgH/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11540 25.06.2025 25.06.2025 Documentos solicitados