Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism (Record no. 45811)
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| fixed length control field | 01733nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140653.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-11595 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Protocols, 2(11), p.2857-2864, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Accurate analysis of DNA sequence variation in not only humans and animals but also other organisms has played a significant role in expanding our knowledge about genetic variety and diversity in a number of different biological areas. The search for an understanding of the causes of genetic variants and mutations has resulted in the development of a simple laboratory technique, known as the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)method, for the detection of single nucleotide polymorphisms (SNPs). PCR-RFLP allows rapid detection of point mutations after the genomic sequences are mplified by PCR. The mutation is discriminated by digestion with specific restriction endonucleases and is identified by gel electrophoresis after staining with ethidium bromide (EtBr). This convenient and simple method is inexpensive and accurate for SNP genotyping and especially useful in small basic research studies of complex genetic diseases. The whole protocol takes only a day to carry out. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ota, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fukushima, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kulski, J.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Inoko, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1-IH358mhFKfgKG--RN2voDnoQtEwi0vb/view?usp=drivesdk">https://drive.google.com/file/d/1-IH358mhFKfgKG--RN2voDnoQtEwi0vb/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-11595 | 25.06.2025 | 25.06.2025 | Documentos solicitados |