Silver staining DNA in polyacrylamide gels (Record no. 45812)

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control field 20250625140653.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11596
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245 10 - TITLE STATEMENT
Title Silver staining DNA in polyacrylamide gels
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Volume/sequential designation Nature Protocols, 2(11), p.2649-2654, 2007
520 3# - SUMMARY, ETC.
Summary, etc. This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis (PAGE). Sensitivity rivals radioisotopic methods and DNA in the picogram range can be reliably detected. The described protocol is fast (B1 h)and is implemented using readily available chemicals and materials. To achieve the sensitivity and visual clarity expected, quality reagents and clean handling are important. The updated protocol described here is based on the widely used method of Bassam et al. (1991), but provides improved image contrast and less risk of staining artefacts.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bassam, B.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gresshoff, P.M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/14vmoMvURDBdCSYyB6qh_6ftGZKrZTuLk/view?usp=drivesdk">https://drive.google.com/file/d/14vmoMvURDBdCSYyB6qh_6ftGZKrZTuLk/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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