A robust plant RNA isolation method suitable for Affymetrix GeneChip analysis and quantitative real-time RT-PCR (Record no. 45814)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11598
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Title A robust plant RNA isolation method suitable for Affymetrix GeneChip analysis and quantitative real-time RT-PCR
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Volume/sequential designation Nature Protocols, 4(3), p.333-340, 2009
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Summary, etc. Microarray analysis and quantitative real-time RT-PCR are the major high-throughput techniques that are used to study transcript profiles. One of the major limitations in these technologies is the isolation of large quantities of highly pure RNA from plant tissues rich in complex polysaccharides, polyphenolics and waxes. Any contamination of the isolated RNA affects the downstream applications and requires extra cleaning procedures that result in a reduced RNA yield, especially the low molecular weight molecules. The protocol presented here is suitable for isolating high yield and clean total RNA from field-grown plants. Unlike current methods, such as LiCl and TRIZOL, with this new method, the isolated RNA can be used directly for Affymetrix GeneChip labeling or real-time RT-PCR without further purification. This fast and simple protocol provides ready-to-use RNA within 4-5 h after sampling. Additionally, the protocol described here maintains the isolation of small RNA molecules, making it an ideal choice for plant RNA preparation prior to high-throughput sequencing methods to study gene expression.
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Personal name Bilgin, D.
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Personal name Delucia, E.H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Clough, S.J.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1FK9QO7corcLRazPHDAZruoaQY2fzCSCX/view?usp=drivesdk">https://drive.google.com/file/d/1FK9QO7corcLRazPHDAZruoaQY2fzCSCX/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11598 25.06.2025 25.06.2025 Documentos solicitados