A Total Extract Dot Blot Hybridization Procedure for mRNA Quantitation in Small Samples of Tissues or Cultured Cells (Record no. 45902)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11686
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245 10 - TITLE STATEMENT
Title A Total Extract Dot Blot Hybridization Procedure for mRNA Quantitation in Small Samples of Tissues or Cultured Cells
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 172(2), p.436-443, 1988
520 3# - SUMMARY, ETC.
Summary, etc. A simple method for the estimation of specific mRNA concentrations in small tissue samples (as little as 1 mg)or cultured cells (lower limit IO5 cells)is described. Guanidine hydrochloride extracts of whole cells or tissues are applied directly onto nitrocellulose and hybridized with the appropriate nick-translated probe. Loading according to DNA content allows expression ofthe result as concentration per cell. Hybridizing with a ribosomal RNA probe allows expression of results relative to rRNA and estimation of the RNA/DNA ratio in the sample. We describe the application of this procedure to the measurement of ceruloplasmin mRNA in tissues and cultured hepatocytes.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RECOMBINANT DNA TECHNOLOGY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MRNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RNA: DNA: GENE EXPRESSION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Grimes, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mcardle, H.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mercer, J.F.B.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1gwe3XIwUi-aNhBUWf2KiNkhVlGc3pnG_/view?usp=drivesdk">https://drive.google.com/file/d/1gwe3XIwUi-aNhBUWf2KiNkhVlGc3pnG_/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11686 25.06.2025 25.06.2025 Documentos solicitados