Molecular fingerprint of bacterial communities and 16S rDNA intra-species heterogeneity: a pitfall that should be considered (Record no. 46020)

MARC details
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fixed length control field 01978nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140657.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11804
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Title Molecular fingerprint of bacterial communities and 16S rDNA intra-species heterogeneity: a pitfall that should be considered
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Volume/sequential designation Pathologie Biologie, 55(8-9), p.434-440, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Molecular fingerprinting methods are currently used to study microbial communities by culture independent approaches. They are proposed as identification tool owing to the availability of rapid automated methods. The 16S rRNA gene (16S rDNA)is an efficient marker for bacterial identification and microbial communities analysis. However, the 16S rDNA polymorphism among strains of the same species is an underestimated pitfall of the fingerprinting approaches. Aim of the study. - We studied the 16S rDNA variability among strains of three bacterial species of medical interest. Material and methods. - Total DNA was extracted from clinical isolates of Pseudomonas aeruginosa (N = 20), Clostridium difficile (N = 20)and Enterobacter cloacae (N = 14). The Polymerase Chain Reaction (PCR)products obtained with consensus primers flanking the 16S rDNA variable regions V3 and V6-V7-V8 were separated by Temporal Temperature Gradient gel Electrophoresis (TTGE). DNA extracted from TTGE bands were sequenced and analysed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ADNR 16S
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR FINGERPRINT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BACTERIAL COMMUNITIES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYMORPHISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BACTERIAL SPECIES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Roudière, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lorto, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tallagrand, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marchandin, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jeannot, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jumas-Bilak, J.L.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1LdkZpR2uOMH2VmIctaxTch4KYBnpcVC5/view?usp=drivesdk">https://drive.google.com/file/d/1LdkZpR2uOMH2VmIctaxTch4KYBnpcVC5/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11804 25.06.2025 25.06.2025 Documentos solicitados