Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava (Record no. 46029)
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| fixed length control field | 01957nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140657.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-11813 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Protocols, 4, p.1845-1854, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Agrobacterium-mediated transformation of friable embryogenic calli (FECEC)is the most widely used method to generate transgenic cassava plants. However, this approach has proven to be time-consuming and can lead to changes in the morphology and quality of FECEC, influencing regeneration capacity and plant health. Here we present a comprehensive, reliable and improved protocol, taking ~6 months, that optimizes Agrobacterium-mediated transformation of FECEC from cassava model cultivar TMS60444. We cocultivate the FECEC with Agrobacterium directly on the propagation medium and adopt the extensive use of plastic mesh for easy and frequent transfer of material to new media. This minimizes stress to the FECEC cultures and permits a finely balanced control of nutrients, hormones and antibiotics. A stepwise increase in antibiotic concentration for selection is also used after cocultivation with Agrobacterium to mature the transformed FECEC before regeneration. The detailed information given here for each step should enable successful implementation of this technology in other laboratories, including those being established in developing countries where cassava is a staple crop. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bull, S.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Owiti, J.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Niklaus, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Beeching, J.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gruissem, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vanderschuren, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/13N-bcW7KMTlXhbSl2eFbE5Y1x1elbisH/view?usp=drivesdk">https://drive.google.com/file/d/13N-bcW7KMTlXhbSl2eFbE5Y1x1elbisH/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-11813 | 25.06.2025 | 25.06.2025 | Documentos solicitados |