Control of microbial activity by flow injection analysis during high cell density cultivation of Escherichia coli (Record no. 46036)

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control field 20250625140658.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11820
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245 10 - TITLE STATEMENT
Title Control of microbial activity by flow injection analysis during high cell density cultivation of Escherichia coli
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Volume/sequential designation Journal of Biotechnology, 27(2), p.143-147, 1993
520 3# - SUMMARY, ETC.
Summary, etc. The application of an automated flow injection analysis (FIA)system for on-line determination of microbial activity, during high cell density cultivations of Escherichia coli is reported. Based on a bioelectrochcmical principle, the FIA method used a redox mediator (potassium hexacyanoferrate(III)) to facilitate electron transfer from the microorganisms to an electrochemical detector. Assays were carried out using a new sampling device which provided aseptic operation by use of a valve and chemical sterilisation. No sample dilution or pretreatment was necessary for biomass concentrations up to approx. 40 g 1-1. The sample volume was 0.5 ml and the overall analysis time was 5 min. FIA signals were found to correlate well with the oxygen uptake rate (OUR). Changes in metabolic activity due to low substrate levels or high inhibitor concentrations in the cultivation medium became obvious from the FIA signals.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ding, T.
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Personal name Bilitewski, U.
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Personal name Schmid, R.D.
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Personal name Korz, D.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sanders, E.A.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1t2jesEPOOYHaKjtVjhUS00OhG4kmL-xe/view?usp=drivesdk">https://drive.google.com/file/d/1t2jesEPOOYHaKjtVjhUS00OhG4kmL-xe/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11820 25.06.2025 25.06.2025 Documentos solicitados