Leishmania parasite detection and quantification sing PCR-ELISA (Record no. 46076)
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| 000 -LEADER | |
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| fixed length control field | 01843nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140658.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-11861 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Leishmania parasite detection and quantification sing PCR-ELISA |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Protocols, 5, p.1074-1080, 2010 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | This protocol describes an improved and optimized PCR -EL ISA method for detection and quantification of Leishmania parasites in host tissues. Unlike other DNA -based assays, this method uses digoxigenin- and biotin-labeled primers. This eliminates the need for a separate step of hybridization of the PCR product with labeled probes. The PCR product is detected using sandwich EL ISA with antidigoxigenin-detecting antibodies. Primers are complementary to the kinetoplast minicircle conserved region of parasite DNA , allowing the detection of several Leishmania species. For measurement of a wide range of parasite concentrations, ±25 cycles were optimal. The sensitivity of this technique is 0.3 fg of parasite DNA per reaction in 40-cycle PCR -EL ISA , corresponding to 0.004 parasites. DNA preparation by a standard TR I reagent procedure takes about 4 h. When DNA is prepared, a single person can test a large number of samples (at least 150)in a maximum of 7 h. This method might also be suitable for detecting and quantifying other pathogens, especially for detecting small di fferences in pathogen numbers. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kobets, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Badalová, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Grekov, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Havelková, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Svobodová, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lipoldová, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1xx67URoB50xAcQ1Z_4jmuF_JRuv_pHya/view?usp=drivesdk">https://drive.google.com/file/d/1xx67URoB50xAcQ1Z_4jmuF_JRuv_pHya/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-11861 | 25.06.2025 | 25.06.2025 | Documentos solicitados |