Studies on preparation and viability of Phytophthora parasitica spheroplasts (Record no. 46172)

MARC details
000 -LEADER
fixed length control field 01663nam a2200193Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140700.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11959
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Studies on preparation and viability of Phytophthora parasitica spheroplasts
490 0# - SERIES STATEMENT
Volume/sequential designation Trans. Br. Mycol. Soc, 89(2), p.213-220, 1987
520 3# - SUMMARY, ETC.
Summary, etc. Spheroplasts were obtained from Phytophthora parasitica mycelium by digestion with Novozym 234 using 0'35 M CaCI 2 (pH 5'7)as initial osmotic stabilizer. Spheroplast yield decreased rapidly with increasing age of the mycelium. Young mycelium gave highest yields of9'3 ±4'5 x 106 cells per Petri dish. Regeneration was investigated on synthetic and complex media in the presence of different osmotic stabilizers. Survival on solid and in liquid media was enhanced in the presence of CaCI2 in addition to mannitol as osmotic stabilizer. When spheroplasts were spread on agar containing 0'1 M CaCI 2 plus 0"4 M mannitol as osmotica the regeneration rate was 12 ± 4 x cent. After embedding in soft agarose stabilized with o-8 M mannitol spheroplast regeneration was 19 ± 6 x pent. Incubation in osmotically stabilized liquid medium resulted in a rapid loss of viability. High concentrations of CaCI 2 as osmotic stabilizer caused abnormal growth of spheroplasts which eventually gave rise to normal mycelium.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jahnke, K.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leipoldt, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Prell, H.H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1EZqGpLTJ4AVxfo4d0pJ71wfwNDmxsIF6/view?usp=drivesdk">https://drive.google.com/file/d/1EZqGpLTJ4AVxfo4d0pJ71wfwNDmxsIF6/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11959 25.06.2025 25.06.2025 Documentos solicitados