Determination of the fraction of S-phase cells in root meristems using bromodeoxyuridine labeling (Record no. 46217)

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control field MX-MdCICY
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control field 20250625140701.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12004
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245 10 - TITLE STATEMENT
Title Determination of the fraction of S-phase cells in root meristems using bromodeoxyuridine labeling
490 0# - SERIES STATEMENT
Volume/sequential designation Biotechnic & HistoChemistry, 77(3)P.145-152, 2002
520 3# - SUMMARY, ETC.
Summary, etc. The use of bromodeoxyuridine (BrdU)and subsequent immunocytochemical visualization for studying cell proliferation in plant meristems was investigated in Allium cepa L. root-tips. We describe the optimization of an indirect immunoperoxidase method for detecting incorporation of this DNA precursor in pulse-labeled cells. The basic object of this study is to quantify the extent to which the fraction of S-phase cells can reliably be estimated in asynchronous populations. A matrix of parallel labeling schedules with tritiated-thymidine or BrdU was developed, and the labeling indices provided by autoradiography or immunocytochemistry were compared. Thus, 0.5 mM BrdU assured saturation S-phase labeling after an exposure time of 30 min, and the mean length of the S-phase determined under such conditions was similar to that previously reported for this plant system. Interestingly, Feulgen staining did not interfere with subsequent detection of the BrdU probe. This allowed comparative evaluations of the nuclear DNA content by Feulgenmicrodensitometry and the position of a given cell in G1, S or G2 compartments. We also explored the possibility of quantifying BrdU-incorporation in single nuclei by densitometry measurement of the peroxidase label.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALLIUM CEPA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BROMODEOXYURIDINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL CYCLE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMMUNOPEROXIDASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MERISTEMS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICRODENSITOMETRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element S-PHASE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hervas, Jp
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Flor, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Santa-Cruz, M.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1oYruqEFDqO9NCzyW3ujGUhp__dHsIx0u/view?usp=drivesdk">https://drive.google.com/file/d/1oYruqEFDqO9NCzyW3ujGUhp__dHsIx0u/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12004 25.06.2025 25.06.2025 Documentos solicitados