Enzyme Kinetics Determined Using Calorimetry: A General Assay for Enzyme Activity? (Record no. 46390)

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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12179
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Title Enzyme Kinetics Determined Using Calorimetry: A General Assay for Enzyme Activity?
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 296(2), p.179-187, 2001
520 3# - SUMMARY, ETC.
Summary, etc. Two techniques for determining enzyme kinetic constants using isothermal titration microcalorimetry are presented. The methods are based on the proportionality between the rate of a reaction and the thermal power (heat/time)generated. (i)An enzyme can be titrated with increasing amounts of substrate, while pseudo-first-order conditions are maintained. (ii)Following a single injection, the change in thermal power as substrate is depleted can be continuously monitored. Both methods allow highly precise kinetic characterization in a single experiment and can be used to measure enzyme inhibition. Applicability is demonstrated using a representative enzyme from each EC classification, including (i)oxidation-reduction activity of DHFR (EC 1.5.1.3); (ii)transferase activity of creatine phosphokinase (EC 2.7.3.2)and hexokinase (EC 2.7.1.1); (iii)hydrolytic activity of Heliobacter pylori urease (EC 3.5.1.5), trypsin (EC 3.4.21.4), and the HIV-1 protease (EC 3.4.21.16); (iv)lyase activity of heparinase (EC 4.1.1.7); and (v)ligase activity of pyruvate carboxylate (EC 6.4.1.1). This nondestructive method is completely general, enabling precise analysis of reactions in spectroscopically opaque solutions, using physiological substrates. Such a universal assay may have wide applicability in functional genomics.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Todd, M.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gomez, J.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1fav1bL9-Ga24DPPHN-42UiFfdJBBI1A_/view?usp=drivesdk">https://drive.google.com/file/d/1fav1bL9-Ga24DPPHN-42UiFfdJBBI1A_/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12179 25.06.2025 25.06.2025 Documentos solicitados