Biosynthesis of b-sitosterol and stigmasterol proceeds exclusively via the mevalonate pathway in cell suspension cultures of Croton stellatopilosus (Record no. 46431)

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control field 20250625140705.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12220
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245 10 - TITLE STATEMENT
Title Biosynthesis of b-sitosterol and stigmasterol proceeds exclusively via the mevalonate pathway in cell suspension cultures of Croton stellatopilosus
490 0# - SERIES STATEMENT
Volume/sequential designation Tetrahedron Letters, 49(25), p.4067-4072, 2008
520 3# - SUMMARY, ETC.
Summary, etc. Cell suspension cultures of Croton stellatopilosus were fed with [1-13C]glucose and [2-13C]sodium acetate and cultured under control conditions. b-Sitosterol and stigmasterol were isolated and their 13C-labeling patterns examined using quantitative NMR spectroscopy. Analysis of the patterns of 13C-enrichment revealed that all the isoprene units in the molecules of both phytosterols originated exclusively from the mevalonate pathway. These results were in contrast with our previous study using callus cultures of C. stellatopilosus, which showed that the isoprene units of b-sitosterol and stigmasterol were supplied equally from both the deoxyxylulose phosphate (DXP)pathway and the mevalonate pathway. Observation by transmission electron microscopy of sub-cellular structures of both cell types revealed that the callus cells contained partially differentiated chloroplasts, whereas the suspension cultured cells did not. Since the DXP pathway is known to be located in the chloroplasts, it was suggested that the presence of chloroplasts is essential for expression of the DXP pathway. Therefore, the sole operation of the phytosterol biosynthesis by the mevalonate pathway observed in this study was likely to be the result of non-expression of the DXP pathway in the chloroplast-free cell suspension cultures of C. stellatopilosus.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CROTON STELLATOPILOSUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL SUSPENSION CULTURE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element B-SITOSTEROL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element STIGMASTEROL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FEEDING EXPERIMENTS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THE MEVALONATE PATHWAY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kongduang, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wungsintaweekul, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De-Eknamkul, W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1lGE9JH6np5dBTULwPijmOgL_obtofYcO/view?usp=drivesdk">https://drive.google.com/file/d/1lGE9JH6np5dBTULwPijmOgL_obtofYcO/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12220 25.06.2025 25.06.2025 Documentos solicitados