MARC details
| 000 -LEADER |
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| fixed length control field |
02060nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625140705.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-12234 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Virological Methods, 143(2), p.132-139, 2007 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The genetic study of double-stranded (ds)RNA viruses by sequence analyses of full-length genome segments, or entire viral genomes, has been restricted by the technical difficulties involved in analyses of dsRNA templates. This paper describes improved methods for sequence-independent synthesis of full-length cDNA copies of dsRNA genes and associated sequencing strategies. These methods include an improved version of the 'Single Primer Amplification Technique' (SPAT - [Attoui, H., Billoir, F., Cantaloube, J.F., Biagini, P., de Micco, P. and de Lamballerie, X., 2000. Strategies for the sequence determination of viral dsRNA genomes. J. Virol. Methods 89, 147-158]), which is described here as 'Full-Length Amplification of cDNAs' (FLAC). They also include the development of direct sequencing methods (without cloning)for the resulting full-length cDNAs. These techniques, which are applicable to any viruses with segmented dsRNA genomes and conserved RNA termini, make it possible to generate sequence data rapidly from multiple isolates for molecular epidemiology studies. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BLUETONGUE VIRUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
REOVIRUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DSRNA VIRUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CDNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RAPID SEQUENCING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FLAC |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Maan, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rao, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Singh Maan, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Anthony, S.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Attoui, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Richard Samuel, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Clement Mertens, P.P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1A8Afrihvr6WCUtYc-IUHoHr-fbl1QDay/view?usp=drivesdk">https://drive.google.com/file/d/1A8Afrihvr6WCUtYc-IUHoHr-fbl1QDay/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
