Changes of cytosolic Ca2þ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress (Record no. 46448)

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fixed length control field 02794nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140705.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12237
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245 10 - TITLE STATEMENT
Title Changes of cytosolic Ca2þ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Physiology and BioChemistry, 48(10-11), p.860-865, 2010
520 3# - SUMMARY, ETC.
Summary, etc. The changes of cytosolic Ca2þ fluorescence intensity and the activities of calcium channel of primary maize root tip cells induced by PEG6000 or abscisic acid(ABA)were studied by both confocal techniques and the whole-cell patch clamping in this study. The Ca2þ fluorescence intensity increased while treated with PEG or ABA within 10 min, illuminating that Ca2þ participated in the process of ABA signal transduction. For further proving the mechanism and origin of cytosolic Ca2þ increase induced by PEG treatments, N,N,N0,N0-tetraacetic acid (EGTA), Verapamil (VP)and Trifluoperazine (TFP)were added to the PEG solution in the experiments separately. The results showed that Ca2þ fluorescence intensity induced by PEG was suppressed by both EGTA and VP obviously in the root tip cells. The Ca2þ fluorescence intensity of plants changed after the addition of CaM inhibitor TFP while subjected to osmotic stress, which seemed to show that CaM participated in the process of signal transduction of osmotic stress too. The mechanism about it is unknown today. Further, a hyperpolarization-activated calcium permeable channel was recorded in plasma membrane of maize root tip cells. The Ca2þ current (ICa)intensity increased remarkably after PEG treatment, and the open voltage of the calcium conductance increased. Similar changes could be observed after ABA treatment, but the channel opened earlier and the current intensity was stronger than that of PEG treatment. The activation of calcium channel initiated by PEG strongly was inhibited by EGTA, VP or TFP respectively. The results revealed that Ca2þ participated in the signals transduction process of osmotic stress, and the cytosolic free Ca2þ increase by osmotic stress mainly came from the extracellular, and some came from the release of cytoplasmic calcium pool.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ABSCISIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MAIZE ROOT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CALCIUM CHANNEL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CONFOCAL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MEMBRANE-CLAMP
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CALCIUM SIGNAL
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liu, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ma, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Guo, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shao, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cui, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Song, W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1DcT1QyU_4hgqfOaTw4R6Rub-dQBNLheI/view?usp=drivesdk">https://drive.google.com/file/d/1DcT1QyU_4hgqfOaTw4R6Rub-dQBNLheI/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12237 25.06.2025 25.06.2025 Documentos solicitados