MARC details
| 000 -LEADER |
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| fixed length control field |
01944nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625140706.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-12255 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Analytical BioChemistry, 343(2), p.313-321, 2005 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
We describe here the construction of a 10-Gateway-based vector set applicable for high-throughput cloning and for expressing recombinant proteins in Escherichia coli. Plasmids bear elements required to produce recombinant proteins under control of the T7 promoter and encode diVerent N-terminal partners. Since the vector set is derived from a unique backbone, a consistent comparison of the impact of fusion partner(s)on protein expression and solubility is easily amenable. Finally, a sequence encoding a six-histidine tag has been inserted to be in frame with the cloned open reading frame either at its C terminus or at the N terminus, giving the Xexibility of choosing the six-histidine tag location for further puriWcation. To test the applicability of our vector set, expression and solubility proWle and six-histidine tag accessibility have been demonstrated for two Bacillus subtilis signaling proteins' encoding genes (SBGP codes E0508 and E0511). |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CLONING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FUSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GATEWAY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HIGH-THROUGHPUT SCREENING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RECOMBINANT PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RECOMBINATIONAL CLONING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
STRUCTURAL GENOMICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TAGS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Busso, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Delagoutte-Busso, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Moras, D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1m0K6rX8DGa3nmeu8Ak5eeAhnL10mtnwK/view?usp=drivesdk">https://drive.google.com/file/d/1m0K6rX8DGa3nmeu8Ak5eeAhnL10mtnwK/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
