Construction of two Gateway vectors for gene expression in fungi (Record no. 46471)
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| fixed length control field | 01844nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140706.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-12260 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Construction of two Gateway vectors for gene expression in fungi |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plasmid, 62(2), p.128-133, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We report the construction of two Gateway fungal expression vectors pCBGW and pGWBF. The pCBGW was generated by introducing an expression cassette, which consists of a Gateway recombinant cassette (attR1-Cmr-ccdB-attR2)under the control of fungal promoter PgpdA and a terminator TtrpC, into the multiple cloning site of fungal vector pCB1004. The pGWBF is a binary vector, which was generated from the plant expression vector pGWB2 by replacing the CaMV35S promoter with PgpdA. The pGWBF can be transformed into fungi efficiently with Agrobacterium-mediated transformation. The applicability of two newly constructed vectors was tested by generating the destination vectors pGWBF-GFP and pCBGW-GFP and examining the expression of GFP gene in Trichoderma viride and Gibberella fujikuroi, respectively. Combining with the advantage of Gateway cloning technology, pCBGW and pGWBF will be useful in fungi for large-scale investigation of gene functions by constructing the interested gene destination/expression vectors in a highthroughput way |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FUNGAL EXPRESSION VECTOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GATEWAY CLONING TECHNOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AGROBACTERIUM-MEDIATED TRANSFORMATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cui, Z. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1hKtyL-T2fdN5WJbWYfb1OnJCtvmg1PuS/view?usp=drivesdk">https://drive.google.com/file/d/1hKtyL-T2fdN5WJbWYfb1OnJCtvmg1PuS/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-12260 | 25.06.2025 | 25.06.2025 | Documentos solicitados |