MARC details
| 000 -LEADER |
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| fixed length control field |
02923nam a2200337Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153850.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-12277 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Bacterial DNA Methylation and Gene Transfer Efficiency |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
BioChemical and Biophysical Research Communications, 276(3), p.1261-1264, 2000 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The necessary amplification step in bacteria of any plasmid currently used in DNA immunization or gene therapy introduces modification in the nucleotide sequence of plasmid DNA used in gene transfer. These changes affect the adenine and the internal cytosine in respectively all of the GATC and CC(A/T)GG sequences. These modifications which introduce 6-methyladenine and 5-methylcytosine in plasmidic DNA are the consequence of the existence of the bacterial modification systems Dam and Dcm. In eucaryotes, the presence of 5-methylcytosine at dinucleotides -CG- is involved in silencing gene expression, but the possible consequences of the presence of the bacterial GmATC and CmC(A/T)GG sequences in the plasmids used in gene transfer experiments are presently unknown. Since the possibility exists to obtain plasmid DNA lacking this specific bacterial pattern of methylation by using (dam2, dcm2)bacteria we performed experiments to compare in vitro and in vivo gene transfer efficiency of a pCMV-luc reporter plasmid amplified either in the JM109 (dam1, dcm1)or JM110 (dam2, dcm2)bacteria. Data obtained demonstrated that the presence of 6-methyladenine in GATC sequences and 5-methylcytosine in the second C of CC(A/T)GG motifs does not reduce the levels of luciferase activity detected following in vitro or in vivo gene transfer. On the contrary, gene transfer with a pCMV-luc amplified in JM109 (dam1, dcm1)bacteria gives greater amounts of luciferase than the same transfection performed with a plasmid amplified in the mutated JM110 (dam2, dcm2)counterpart. Therefore, these data do not suggest that the use of (dam2, dcm2)bacteria to amplify plasmid DNA may increase gene transfer efficiency. However, the persistence of the use of (dam1, dcm1)bacteria in order to amplify plasmid DNA raises the question of the possible biological consequences of the introduction of the bacterial GmATC and CmC(A/T)GG sequences in eukaryotic cells or organisms. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLASMID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BACTERIA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DAM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DCM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENE THERAPY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PATENT. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Allamane, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jourdes, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ratel, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Vicat, J.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dupre, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Laine, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Berger, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Berger, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wion, D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1y6oCN_rCcvptf8yhS4ZlGOZPgJEvXy_r/view?usp=drivesdk">https://drive.google.com/file/d/1y6oCN_rCcvptf8yhS4ZlGOZPgJEvXy_r/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
