Variations in DNA methylation in Picea omorika (Panč) Purk. embryogenic tissue and the ability for embryo maturation (Record no. 46488)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153850.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12278
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245 10 - TITLE STATEMENT
Title Variations in DNA methylation in Picea omorika (Panč) Purk. embryogenic tissue and the ability for embryo maturation
490 0# - SERIES STATEMENT
Volume/sequential designation Propagation of Ornamental Plants, 9(1), p.3-9, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Developmental changes in response to phytohormone treatments are commonly accompanied by variations in DNA methylation. To improve somatic embryo maturation in Picea omorika, a long-term embryogenic tissue line was exposed, for one to four weeks, to a variety of auxin/cytokinin ratios, or to hormone-free medium, before placement onto a maturation medium containing 40 ìM ABA. The extent of cytosine methylation in thus pretreated explants was estimated with the aim to reveal possible interdependence of the global DNA methylation status and the ability for subsequent embryo maturation on ABA-containing medium. The differences in the electrophoretic pattern of RAPD bands following DNA digestion with HpaII and MspI were the criteria used for evaluating the levels of DNA methylation. The latter varied depending on the exogenous hormone supply. Reduction of the 2,4-D level or its omission from the culture medium resulted in decreased methylation. A one-week exposure to hormone-free medium supplemented with activated charcoal coincided with reduced DNA methylation and improved subsequent somatic embryo maturation on the ABA-containing medium. A similar reduction in DNA methylation was observed after one-week exposure to 5-azaC in the presence of 2,4-D and BA, but this treatment did not affect subsequent embryo development.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 5-AZACYTIDINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA METHYLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MATURATION ABILITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PICEA OMORIKA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leljak-Levanic, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mihaljevic, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jelaska, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/113VskC3k58XqIm-WKCzuG6W4JHcODysZ/view?usp=drivesdk">https://drive.google.com/file/d/113VskC3k58XqIm-WKCzuG6W4JHcODysZ/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12278 25.06.2025 25.06.2025 Documentos solicitados