MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02283nam a2200349Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625153850.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-12284 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: Do the different methods provide similar results? |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Journal of MicroBiological Methods, 69(3), p.470-479, 2007 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Bacterial communities of four arable soils - pelosol, gley, para brown soil, and podsol brown soil - were analysed by fingerprinting of 16S rRNA gene fragments amplified from total DNA of four replicate samples for each soil type. Fingerprints were generated in parallel by denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), and single strand conformation polymorphism (SSCP)to test whether these commonly applied techniques are interchangeable. PCR amplicons could be separated with all three methods resulting in complex ribotype patterns. Although the fragments amplified comprised different variable regions and lengths, DGGE, T-RFLP and SSCP analyses led to similar findings: (a)a clustering of fingerprints which correlated with soil physico-chemical properties, (b)little variability between the four replicates of the same soil, (c)the patterns of the two brown soils were more similar to each other than to those of the other two soils, and (d)the fingerprints of the different soil types revealed significant differences in a permutation test, which was recently developed for this purpose. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
16S RRNA GENE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
DGGE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SOIL BACTERIAL DIVERSITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SSCP |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
TOTAL COMMUNITY DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
T-RFLP |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Smalla, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Oros-Sichler, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Milling, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Heuer, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Baumgarte, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Becker, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Neuber, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Neuber, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ulrich, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Tebbe, C.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1nxTZaDuY5hvuOjFQLpBlHeFaif3XrsPQ/view?usp=drivesdk">https://drive.google.com/file/d/1nxTZaDuY5hvuOjFQLpBlHeFaif3XrsPQ/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
