CsCCD2 Access Tunnel Design for a Broader Substrate Profile in Crocetin Production (Record no. 46497)

MARC details
000 -LEADER
fixed length control field 02273nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153850.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12287
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title CsCCD2 Access Tunnel Design for a Broader Substrate Profile in Crocetin Production
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Agricultural and Food Chemistry, 69(39), p.11626-11636, 2021
520 3# - SUMMARY, ETC.
Summary, etc. Crocetin, a high-value apocarotenoid in saffron, is widely applied to the fields of food and medicine. However, the existing method of obtaining crocetin through large-scale cultivation is far from meeting the market demand. Microbial synthesis of crocetin is a potential alternative to traditional resources, and it is found that carotenoid cleavage dioxygenase (CCD)is the critical enzyme to synthesize crocetin. So, in this study, we used "hybrid-tunnel" engineering to obtain variants of Crocus sativus-derived CsCCD2, essential for zeaxanthin conversion into crocetin, with a broader substrate specificity and higher catalytic efficiency. Variants including S323A, with a lower charge bias and a larger tunnel size than the wild-type, showed a 5-fold higher crocetin titer in yeast-based fermentations. S323A could also convert the ?-carotene substrate to crocetin dialdehyde and exhibited a 12.83-fold greater catalytic efficiency (kcat/Km)toward zeaxanthin than the wild-type in vitro. This strategy enabled the production of 107 mg/L crocetin in 5 L fed-batch fermentation, higher than that previously reported. Our findings demonstrate that engineering access tunnels to expand the substrate profile by in silico protein design represents a viable strategy to refine the catalytic properties of enzymes across a range of applications.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CROCETIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CCD2
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ACCESS TUNNEL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENGINEERING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SACCHAROMYCES CEREVISIAE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liang, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yao, M. D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liu, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Feng, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, Z. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yuan, Y. J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1V18cw3FxEyDWbqPJROqkMtdCifhfFWyX/view?usp=drivesdk">https://drive.google.com/file/d/1V18cw3FxEyDWbqPJROqkMtdCifhfFWyX/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12287 25.06.2025 25.06.2025 Documentos solicitados