A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen (Record no. 46501)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153850.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12291
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245 10 - TITLE STATEMENT
Title A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Pathology, 59(2), p.348-357, 2010
520 3# - SUMMARY, ETC.
Summary, etc. This study analysed genomic variation of the translation elongation factor 1a (TEF-1a)and the intergenic spacer region (IGS)of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc)isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR-based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FUSARIUM OXYSPORUM F. SP. CUBENSE IN PLANTA DETECTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MUSA SPP.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PANAMA DISEASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR-BASED DIAGNOSTIC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VEGETATIVE COMPATIBILITY GROUPS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dita, M.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Waalwijk, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Buddenhagen, I.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Souza Jr, M.T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kema, G.H.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/102U4nGSU0tNF3QU3HEA5hPaC7v7w028n/view?usp=drivesdk">https://drive.google.com/file/d/102U4nGSU0tNF3QU3HEA5hPaC7v7w028n/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12291 25.06.2025 25.06.2025 Documentos solicitados