Differential gene expression related to an epigenetic memory affecting climatic adaptation in Norway spruce (Record no. 46599)

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fixed length control field 02472nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153852.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12391
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245 10 - TITLE STATEMENT
Title Differential gene expression related to an epigenetic memory affecting climatic adaptation in Norway spruce
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science, 180(1), p.132-139, 2011
520 3# - SUMMARY, ETC.
Summary, etc. In Norway spruce, the temperature during zygotic embryogenesis appears to adjust an adaptive epigenetic memory in the progeny that may regulate bud phenology and cold acclimation. Conditions colder than normal advance the timing whilst temperatures above normal delay the onset of these processes and altered performance is long lasting in progeny with identical genetic background. As a step toward unraveling the molecular mechanism behind an epigenetic memory, transcriptional analysis was performed on seedlings from seeds of six full-sib families produced at different embryogenesis temperature-cold (CE)vs warm (WE)under long and short day conditions. We prepared two suppressive subtractedcDNAlibraries, forward and reverse, representing genes predominantly expressed in plants from seeds obtained after CE and WE embryogenesis following short day treatment (inducing bud set). Sequencing and annotation revealed considerable differences in the transcriptome of WE versus CE originated plants. By using qRT-PCR we studied the expression patterns of 32 selected candidate genes chosen from subtractive cDNA libraries analysis and nine siRNA pathways genes by a direct candidate approach. Eight genes, two transposons related genes, three with no match to Databases sequences and three genes from siRNA pathways (PaDCL1 and 2, PaSGS3)showed differential expression in progeny from CE andWEcorrelated with the family phenotypic differences. These findingsmaycontribute to our understanding of the epigenetic mechanisms underlying adaptive changes acquired during embryogenesis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EPIGENETIC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BUD SET
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUBTRACTED LIBRARIES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENE EXPRESSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PICEA ABIES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yakovlev, I.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Asante, D.K.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fossdal, C.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Junttila, O.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Johnsen, O.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1nuqJcUV0GmnzTlaDlUPti_D21MIkiC8X/view?usp=drivesdk">https://drive.google.com/file/d/1nuqJcUV0GmnzTlaDlUPti_D21MIkiC8X/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12391 25.06.2025 25.06.2025 Documentos solicitados