Genetically pyramiding protease-inhibitor genes for dual road-spectrum resistance against insect and phytopathogens in transgenic tobacco (Record no. 46706)

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fixed length control field 01989nam a2200277Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153855.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12499
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245 10 - TITLE STATEMENT
Title Genetically pyramiding protease-inhibitor genes for dual road-spectrum resistance against insect and phytopathogens in transgenic tobacco
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Biotechnology Journal, 8(1), p.65-75, 2010
520 3# - SUMMARY, ETC.
Summary, etc. Protease inhibitors provide a promising means of engineering plant resistance against attack by insects and pathogens. Sporamin (trypsin inhibitor)from sweet potato and CeCPI (phytocystatin)from taro were stacked in a binary vector, using pMSPOA (a modified sporamin promoter)to drive both genes. Transgenic tobacco lines of T0 and T1 generation with varied inhibitory activity against trypsin and papain showed resistance to both insects and phytopathogens. Larvae of Helicoverpa armigera that ingested tobacco leaves either died or showed delayed growth and development relative to control larvae. Transgenic tobacco-overexpressing the stacked genes also exhibited strong resistance against bacterial soft rot disease caused by Erwinia carotovora and damping-off disease caused by Pythium aphanidermatum. Thus, stacking protease-inhibitor genes, driven by the wound and pathogen responsive pMSPOA promoter, is an effective strategy for engineering crops to resistance against insects and phytopathogens.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SPORAMIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CECPI (TARO CYSTATIN)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SYNTHETIC WOUND-INDUCIBLE&PROMOTER (PMSPOA)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSGENIC TOBACCO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INSECT RESISTANCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTIBACTERIAL ACTIVITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTIFUNGAL ACTIVITY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Senthilkumar, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cheng, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yeh, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Ela5eGXdmJXX1d2Y7CVTl2K2Nvu6oXT6/view?usp=drivesdk">https://drive.google.com/file/d/1Ela5eGXdmJXX1d2Y7CVTl2K2Nvu6oXT6/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12499 25.06.2025 25.06.2025 Documentos solicitados