Single-strand conformation polymorphism (SSCP)for the analysis of genetic variation (Record no. 46713)
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| 000 -LEADER | |
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| fixed length control field | 02160nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153856.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-12506 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Single-strand conformation polymorphism (SSCP)for the analysis of genetic variation |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Protocols, 1(6), p.3121-3128, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The accurate analysis of genetic variation has major implications in many areas of biomedical research, including the identification of infectious agents (such as parasites), the diagnosis of infections, and the detection of unknown or known disease-causing mutations. Mutation scanning methods, including PCR-coupled single-strand conformation polymorphism (SSCP), have significant advantages over many other nucleic acid techniques for the accurate analysis of allelic and mutational sequence variation. The present protocol describes the SSCP method of analysis, including all steps from the small-scale isolation of genomic DNA and PCR amplification of target sequences, through to the gel-based separation of amplicons and scanning for mutations by SSCP (either by the analysis of radiolabeled amplicons in mutation detection enhancement (MDE)gels or by non-isotopic SSCP using precast GMA gels). The subsequent sequence analysis of polymorphic bands isolated from gels is also detailed. The SSCP protocol can readily detect point mutations for amplicon sizes of up to 450-500 bp, and usually takes 1-2 days to carry out. This user-friendly, low-cost, potentially high-throughput platform has demonstrated the utility to study a wide range of pathogens and diseases, and has the potential to be applied to any gene of any organism. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gasser, R.B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hu, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chilton, N.B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Campbell, B.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jex, A.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Otranto, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cafarchia, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cafarchia, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, X. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1T_VM6QNnyLX9tcwr3ZQELtg1ed36OQkv/view?usp=drivesdk">https://drive.google.com/file/d/1T_VM6QNnyLX9tcwr3ZQELtg1ed36OQkv/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-12506 | 25.06.2025 | 25.06.2025 | Documentos solicitados |