Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1 (Record no. 46721)

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control field 20250625153856.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12514
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245 10 - TITLE STATEMENT
Title Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 269(2), p.337-341, 1999
520 3# - SUMMARY, ETC.
Summary, etc. A spectrophotometric method has been developed for the quantitative determination of antioxidant capacity. The assay is based on the reduction of Mo(VI)to Mo(V)by the sample analyte and the subsequent formation of a green phosphate/Mo(V)complex at acidic pH. The method has been optimized and characterized with respect to linearity interval, repetitivity and reproducibility, and molar absorption coefficients for the quantitation of several antioxidants, including vitamin E. The phosphomolybdenum method, in combination with hexane monophasic extraction, has also been adapted for the specific determination of vitamin E in seeds. The results obtained with the proposed method were validated by comparison with a standard HPLC method. The phosphomolybdenum method is routinely applied in our laboratory to evaluate the total antioxidant capacity of plant extracts and to determine vitamin E in a variety of grains and seeds, including corn and soybean.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Prieto, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pineda, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Aguilar, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1FnX-mDeFtX5uQXkOM3aV9a2Cy4NASHnI/view?usp=drivesdk">https://drive.google.com/file/d/1FnX-mDeFtX5uQXkOM3aV9a2Cy4NASHnI/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12514 25.06.2025 25.06.2025 Documentos solicitados