Detection of phytoplasmas in dieback, yellow crinkle, and mosaic diseases of papaya using polymerase chain react ion techniques (Record no. 46728)

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control field 20250625153856.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12521
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245 10 - TITLE STATEMENT
Title Detection of phytoplasmas in dieback, yellow crinkle, and mosaic diseases of papaya using polymerase chain react ion techniques
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Volume/sequential designation Australian Journal of agricultural Research, 47(3), p.387-394, 1996
520 3# - SUMMARY, ETC.
Summary, etc. Oligonucleotide primers complementary to regions specific to plant-pathogenic mycoplasma-like organisnls (phytoplasmas)were used in polymerase chain reactions on tissue samples from dieback, yellow crinkle, and mosaic affected papaya plants. The primer pair P068/P069, which hybridise to internal regions of the 16s rRNA gene, amplified an approximately 560 bp product in dieback, yellow crinkle and mosaic affected papaya. The primer pair P3/P7, which hybridise to the spacer region between the 16s and 23s rRNA genes. amplified an approximately 300 bp fragment in yellow crinkle and mosaic affected papaya, with no product from dieback affected plants. No PCR product was obtained with either set of primers from healthy plants. An identical AluI restriction enzyme profile was obtained with all three 560 bp products. This study provides the first evidence for the association of phytoplasmas with papaya mosaic and Australian papaya dieback.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARICA PAPAYA, PAPAYA
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Topical term or geographic name entry element DIEBACK
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Topical term or geographic name entry element PAPAYA YELLOW CRINKLE
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Topical term or geographic name entry element PAPAYA MOSAIC
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Topical term or geographic name entry element PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHYTOPLASMAS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MYCOPLASMA-LIKE ORGANISMS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liu, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name White, D.T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Walsh, K.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Scott, P.T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1OvJFawoE6dTooKkC9Rgz3GL8curbfXqX/view?usp=drivesdk">https://drive.google.com/file/d/1OvJFawoE6dTooKkC9Rgz3GL8curbfXqX/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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