Characterization and partial purification of dl-glycerol-1-phosphatase from Dunaliella salina (Record no. 46862)

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000 -LEADER
fixed length control field 01492nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
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control field 20250625153904.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12655
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245 10 - TITLE STATEMENT
Title Characterization and partial purification of dl-glycerol-1-phosphatase from Dunaliella salina
490 0# - SERIES STATEMENT
Volume/sequential designation Biochimica et Biophysica Acta, 661(2), p.199-204, 1981
520 3# - SUMMARY, ETC.
Summary, etc. A specific DL-glycerol-l-phosphatase (glycerol-l-phosphate phosphohydrolase, EC 3.1.3.21)has been identified in the halotolerant alga Duniella salina. The enzyme is highly specific for DL-glycerol 1-phosphate, requires magnesium for activity and has a neutral pH optimum. High sensitivity toward sulfhydryl reagents suggests the existence of a sulfhydryl group in close proximity to the active site. Due to instability the enzyme was only partially purified (40-fold). Activity measurements following polyacrylamide electrophoresis showed the enzyme to have a molecular weight around 86 kdaltons. It is suggested that the enzyme plays a major role in the mechanism of osmoregulation in Dunaliella.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DL-GLYCEROL-L-PHOSPHATASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OSMOREGULATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLYCEROL METABOLISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element (DUNALIELLA SALINA)
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sussman, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Avron, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1PgnZ2bQZ1hIjFMs8T_3ZB2LzFOmfcsTl/view?usp=drivesdk">https://drive.google.com/file/d/1PgnZ2bQZ1hIjFMs8T_3ZB2LzFOmfcsTl/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12655 25.06.2025 25.06.2025 Documentos solicitados