Formation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor (Record no. 46902)

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fixed length control field 01710nam a2200289Ia 4500
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control field MX-MdCICY
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control field 20250625153905.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12695
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245 10 - TITLE STATEMENT
Title Formation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 162(1), p.47-54, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Using a direct somatic embryogenesis system in carrot, we examined the role of DNA methylation in the change of cellular differentiation state, from somatic to embryogenic. 5-Azacytidine (aza-C), an inhibitor of DNA methylation suppressed the formation of embryogenic cell clumps from epidermal carrot cells. Aza-C also downregulated the expression of DcLEC1c, a LEC1-like embryonic gene in carrot, during morphogenesis of embryos. A carrot DNA methyltransferase gene, Met1-5 was expressed transiently after the induction of somatic embryogenesis by 2,4- dichlorophenoxyacetic acid (2,4-D), before the formation of embryogenic cell clumps. These findings suggested the significance of DNA methylation in acquiring the embryogenic competence in somatic cells in carrot.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 5-AZACYTIDINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARROT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA METHYLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yamamoto, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kobayashi, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Togashi, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mori, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kikuchi, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kuriyama, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tokuji, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1D3mVPafMd6zEKOw2YTuV1-qcZLPGQO9s/view?usp=drivesdk">https://drive.google.com/file/d/1D3mVPafMd6zEKOw2YTuV1-qcZLPGQO9s/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12695 25.06.2025 25.06.2025 Documentos solicitados