Formation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor (Record no. 46902)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01710nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153905.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-12695 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Formation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Plant Physiology, 162(1), p.47-54, 2005 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Using a direct somatic embryogenesis system in carrot, we examined the role of DNA methylation in the change of cellular differentiation state, from somatic to embryogenic. 5-Azacytidine (aza-C), an inhibitor of DNA methylation suppressed the formation of embryogenic cell clumps from epidermal carrot cells. Aza-C also downregulated the expression of DcLEC1c, a LEC1-like embryonic gene in carrot, during morphogenesis of embryos. A carrot DNA methyltransferase gene, Met1-5 was expressed transiently after the induction of somatic embryogenesis by 2,4- dichlorophenoxyacetic acid (2,4-D), before the formation of embryogenic cell clumps. These findings suggested the significance of DNA methylation in acquiring the embryogenic competence in somatic cells in carrot. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | 5-AZACYTIDINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CARROT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA METHYLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOGENESIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yamamoto, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kobayashi, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Togashi, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mori, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kikuchi, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kuriyama, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tokuji, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1D3mVPafMd6zEKOw2YTuV1-qcZLPGQO9s/view?usp=drivesdk">https://drive.google.com/file/d/1D3mVPafMd6zEKOw2YTuV1-qcZLPGQO9s/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-12695 | 25.06.2025 | 25.06.2025 | Documentos solicitados |
