MARC details
| 000 -LEADER |
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| fixed length control field |
02334nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153906.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-12701 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Cytosine methylation is associated with RNA silencing in silenced plants but not with systemic and transitive RNA silencing through grafting |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Gene, 396(2), p.321-331, 2007 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
RNA silencing is often associated with methylation of the target gene. The DNA methylation level of transgenes was investigated in posttranscriptionally silenced or non-silenced Nicotiana benthamiana carrying either the 5_O region (200 or 400 bp)or the entire region of the coat protein gene (CP, including the 3_O non-translated region)of Sweet potato feathery mottle virus. Higher levels of transgene cytosine methylation were observed in both symmetrical (CpG, CpNpG)and non-symmetrical (CpHpH)contexts (CpGNCpNpGNCpHpH)in silenced lines, but there was very lower levels or no transgene methylation in non-silenced lines. RNA silencing was induced in non-silenced scions from silenced rootstocks and spread to the 3_O region of the transgene mRNA (Haque et al., Plant Mol. Biol. 2007; 63: 35.47). In this system, transgene methylation levels were analyzed in scions at different time intervals after being grafted onto silenced or non-silenced rootstocks to investigate if transgene methylation was associated with induction or transitivity of RNA silencing. We observed that, there was no change of transgene methylation level in the initial target or in extended regions in scions. These results showed that transgene methylation was associated with RNA silencing in individual transformants, but it was not associated with systemic RNA silencing and/or transitive RNA silencing through grafting. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA-DEPENDENT DNA METHYLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NICOTIANA BENTHAMIANA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
COAT PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SWEET POTATO FEATHERY MOTTLE VIRUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA SILENCING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GRAFTING |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Nazmul Haque, A.K.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yamaoka, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Nishiguchi, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1NPygcNH0wTT8g3JLLrXmkvE6xZiNZqq7/view?usp=drivesdk">https://drive.google.com/file/d/1NPygcNH0wTT8g3JLLrXmkvE6xZiNZqq7/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
