A Second Gene for Acyl-(Acyl-Carrier-Protein): Glycerol-3-Phosphate Acyltransferase in Squash, Cucurbita moschata cv. Shirogikuza*, Codes for an Oleate-Selective Isozyme: Molecular Cloning and Protein Purification Studies (Record no. 46921)
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| fixed length control field | 02044nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153906.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-12714 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A Second Gene for Acyl-(Acyl-Carrier-Protein): Glycerol-3-Phosphate Acyltransferase in Squash, Cucurbita moschata cv. Shirogikuza*, Codes for an Oleate-Selective Isozyme: Molecular Cloning and Protein Purification Studies |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant & Cell Physiology, 41(12), p.1381-1391, 2000 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A new isogene for acyl-(acyl-carrier-protein):glycerol-3-phosphate acyltransferase (GPAT; EC 2.3.1.15)in squash has been cloned and the gene product was identified as oleate- selective GPAT. Using PCR primers that could hybridise with exons for a previously cloned squash GPAT, we obtained two PCR products of different size: one coded for a previously cloned squash GPAT corresponding to non-selective isoforms AT2 and AT3, and the other for a new isozyme, probably the oleate-selective isoform AT1. Fulllength amino acid sequences of respective isozymes were deduced from the nucleotide sequences of genomic genes and cDNAs, which were cloned by a series of PCR-based methods. Thus, we designated the new gene CmATS1;1 and the other one CmATS1;2. Genome blot analysis revealed that the squash genome contained the two isogenes at nonallelic loci. AT1-active fractions were partially purified, and three polypeptide bands were identified as being AT1 polypeptides, which exhibited relative molecular masses of 39.5-40.5 kDa, pI values of 6.75-7.15, and oleate selectivity over palmitate. Partial amino-terminal sequences obtained from two of these bands verified that the new isogene codes for AT1 polypeptides. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nishida, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sugiura, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Enju, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nakamura, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1h2X_GiqDrIQQhj2ro7qQVs911GIrWLnn/view?usp=drive_link">https://drive.google.com/file/d/1h2X_GiqDrIQQhj2ro7qQVs911GIrWLnn/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-12714 | 25.06.2025 | 25.06.2025 | Documentos solicitados |