The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization (Record no. 46949)

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control field MX-MdCICY
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control field 20250625153908.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12742
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Title The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization
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Volume/sequential designation Journal of Biol. Chem., 286(23), p.20431-20440, 2011
520 3# - SUMMARY, ETC.
Summary, etc. The Agrobacterium tumefaciens BlcR is a member of the emerging isocitrate lyase transcription regulators (IclR)that negatively regulates metabolism of ã-butyrolactone, and its repressing function is relieved by succinate semialdehyde (SSA). Our crystal structure showed that BlcR folded into the DNA- and SSA-binding domains, and dimerized via the DNA-binding domains. Mutational analysis identified residues, including F147, that are important for SSA association; BlcRF147A existed as tetramer. Two BlcR dimers bound to target DNA and in a cooperative manner, and distance between the two BlcR-binding sequences in DNA was critical for BlcR-DNA association. Tetrameric BlcRF147A retained DNA-binding activity, and importantly this activity was not affected by distance separating the BlcR-binding sequences in DNA. SSA did not dissociate tetrameric BlcRF147A or BlcRF147A-DNA. Besides in the SSA-binding site, F147 is located in a structurally flexible loop that may be involved in BlcR oligomerization. We propose that SSA regulates BlcR DNAbinding function via oligomerization.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pan, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fiscus, V.
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Personal name Meng, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zheng, Z.
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Personal name Zhang, L.
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Personal name Fuqua, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chen, L.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1yr9Aisk61Ds6lbZ7si2XLQWt6bzuZvbP/view?usp=drivesdk">https://drive.google.com/file/d/1yr9Aisk61Ds6lbZ7si2XLQWt6bzuZvbP/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12742 25.06.2025 25.06.2025 Documentos solicitados