Synthetic Peptides as Antigens" Pitfalls of Conjugation Methods (Record no. 46967)

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control field MX-MdCICY
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control field 20250625153908.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12761
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245 10 - TITLE STATEMENT
Title Synthetic Peptides as Antigens" Pitfalls of Conjugation Methods
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of lmmunological Methods, 78(1), p.59-69, 1985
520 3# - SUMMARY, ETC.
Summary, etc. Peptide-carrier conjugates were prepared using 9 different synthetic peptides, 3 carrier proteins and 4 coupling reagents. Residues of the carrier protein that were modified by different coupling reagents (e.g., glutaraldehyde, carbodiimides, bis-diazotized benzidine)were found to elicit specific antibodies that reacted with unrelated carrier proteins treated with the same coupling agent. To demonstrate the presence of peptide antibodies in an antiserum raised against a peptide-carrier conjugate, it was necessary to use as antigen the peptide coupled to another carrier by means of a different coupling agent. Some of the commonly used conjugation methods were found to lead to conjugates of insufficient stability and sometimes also altered the antigenic properties of the peptide moiety. These difficulties can be overcome by additional control experiments designed to test the quality and the peptide-carrier conjugates.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SYNTHETIC PEPTIDES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COUPLING PROCEDURES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARBODIIMIDES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUTARALDEHYDE-BIS-DIAZOTIZED BENZIDINE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Briand, J.P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Muller, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Van Regenmortel, M,H.V.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/18T-gDnJZdcXRp1__lFz27kTanx4YBx8B/view?usp=drivesdk">https://drive.google.com/file/d/18T-gDnJZdcXRp1__lFz27kTanx4YBx8B/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12761 25.06.2025 25.06.2025 Documentos solicitados