MARC details
| 000 -LEADER |
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| fixed length control field |
01915nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153908.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-12762 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
An Improved Protocol for Coupling Synthetic Peptides to Carrier Proteins for Antibody Production Using DMF to Solubilize Peptides |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of BioMolecular Techniques, 18(3), p.173-176, 2007 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
We present an improved protocol for coupling synthetic peptides to carrier proteins. In this protocol, dimethylformamide is used as the solvent to solubilize peptides instead of phosphate-buffered saline (PBS)or 6 M guanidine-HC l/0.01 M phosphate buffer (pH 7). Additionally, the last desalting or dialyzing step to remove uncoupled peptides as in the traditional method is eliminated. Finally, 3 mL of 0.1 M ammonium bicarbonate is added to the carrier protein conjugated peptide solution to help the lyophilization process. Coupling of Cys-containing synthetic peptides to keyhole limpet hemocyanin or bovine serum albumin using mmaleimidobenzoyl- N-hydroxysuccinimide ester are used as the test cases. This method produces high-quality antipeptide antibodies. Also, compared to the traditional method, this procedure is simpler and useful for peptides with solubility problems in PBS or 6 M guanidine-HC l. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PEPTIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIBODY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
KLH |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BSA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MBS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DMF |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MALDI- TOF MS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Syed Salman Lateef |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Shalini Gupta |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lasanthi P. Jayathilaka |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Sangeeth Krishnanchettiar |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jin-Sheng Huang |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bao-Shiang Lee |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1qQBDR1MayE9oarTAoX-ADGzzSYtE4b2l/view?usp=drivesdk">https://drive.google.com/file/d/1qQBDR1MayE9oarTAoX-ADGzzSYtE4b2l/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
