Crystal Structures of Human Glycerol 3-phosphate Dehydrogenase 1 (GPD1) (Record no. 47015)

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fixed length control field 01845nam a2200325Ia 4500
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control field MX-MdCICY
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control field 20250625153910.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12810
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Title Crystal Structures of Human Glycerol 3-phosphate Dehydrogenase 1 (GPD1)
490 0# - SERIES STATEMENT
Volume/sequential designation J. Mol. Biol., 357(3), p.858-869, 2006
520 3# - SUMMARY, ETC.
Summary, etc. Homo sapiens L-a-glycerol-3-phosphate dehydrogenase 1 (GPD1)catalyzes the reversible biological conversion of dihydroxyacetone (DHAP)to glycerol-3-phosphate. The GPD1 protein was expressed in Escherichia coli, and purified as a fusion protein with glutathione S-transferase. Here we report the apoenzyme structure of GPD1 determined by multiwavelength anomalous diffraction phasing, and other complex structures with small molecules (NADC and DHAP)by the molecular replacement method. This enzyme structure is organized into two distinct domains, the N-terminal eight-stranded b-sheet sandwich domain and the C-terminal helical substrate-binding domain. An electrophilic catalytic mechanism by the 3-NHC 3 group of Lys204 is proposed on the basis of the structural analyses. In addition, the inhibitory effects of zinc and sulfate on GPDHs are assayed and discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element L-A-GLYCEROL-3-PHOSPHATE DEHYDROGENASE 1
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NAD-DEPENDENT DEHYDROGENASE
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Topical term or geographic name entry element CRYSTAL STRUCTURE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CATALYTIC MECHANISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DHAP ACCUMULATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ou, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ji, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Han, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhao, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mao, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wong, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wong, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rao, Z.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1UkI0Bd0_oNXGmuXSdvvfp5Tqux477UB3/view?usp=drivesdk">https://drive.google.com/file/d/1UkI0Bd0_oNXGmuXSdvvfp5Tqux477UB3/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12810 25.06.2025 25.06.2025 Documentos solicitados