GUT2, a Gene for Dehydrogenase of Mitochondrial Saccharomy ces Glycerol 3-Phosphate cerevisiae (Record no. 47044)

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fixed length control field 01808nam a2200217Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153910.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12839
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245 10 - TITLE STATEMENT
Title GUT2, a Gene for Dehydrogenase of Mitochondrial Saccharomy ces Glycerol 3-Phosphate cerevisiae
490 0# - SERIES STATEMENT
Volume/sequential designation Yeast, 9(10), p.1121-1130, 1993
520 3# - SUMMARY, ETC.
Summary, etc. A gut2 mutant of Saccharomyces cerevisiae is deficient in the mitochondrial glycerol 3-phosphate dehydrogenase and hence cannot utilize glycerol. Upon transformation of a gut2 mutant strain with a low-copy yeast genomic library, hybrid plasmids were isolated which complemented the gut2 mutation. The nucleotide sequence of a 3.2 kb PstI-XhoI fragment complementing a gut2 mutant strain is presented. The fragment reveals an open reading frame (ORF)encoding a polypeptide with a predicted molecular weight of 68.8 kDa. Disruption of the ORF leads to a glycerol non-utilizing phenotype. A putative flavin-binding domain, located at the amino terminus, was dentified by comparison with the amino acid sequences of other flavoproteins. The cloned gene has been mapped both physically and genetically to the left arm of chromosome IX, where the original gut2 mutation also maps. We conclude that the presented ORF is the GUT2 gene and propose that it is the structural gene for the itochondrial glycerol 3-phosphate dehydrogenase.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MITOCHONDRIA1 GLYCEROL 3-PHOSPHATE DEHYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLYCEROL UTILIZATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SACCHAROMYCES CEREVISIAE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name R0Nnow, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kielland-Brandt, M.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1_lTu75JrKdxAf7dKwNv38jlxrJYK5n5E/view?usp=drivesdk">https://drive.google.com/file/d/1_lTu75JrKdxAf7dKwNv38jlxrJYK5n5E/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12839 25.06.2025 25.06.2025 Documentos solicitados