Partial Characterization of the Signaling Pathway for the Nitrate-Dependent Expression of Genes for Nitrogen-Assimilatory Enzymes Using Detached Maize Leaves (Record no. 47050)

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control field 20250625153911.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12845
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Title Partial Characterization of the Signaling Pathway for the Nitrate-Dependent Expression of Genes for Nitrogen-Assimilatory Enzymes Using Detached Maize Leaves
490 0# - SERIES STATEMENT
Volume/sequential designation Plant & Cell Physiology, 38(7), p.837-843, 1997
520 3# - SUMMARY, ETC.
Summary, etc. We have studied the regulation of the nitrate-dependent expression of genes for the nitrogen-assimilatory enzymes, nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS)and glutamate synthase (GOGAT), using detached leaves of maize plants that were grown with a limited supply of nitrate. In the detached leaves, transcripts of genes for NR, NiR, plastidic GS (GS2), ferredoxin- GOGAT (Fd-GOGAT)and NADH-GOGAT accumulated upon treatment of detached leaves with nitrate. The kinetics of accumulation was similar for all transcripts but the amount accumulated was smaller for the transcripts of genes for both GOGATs. Experiments with cordycepin and cycloheximide suggested that, in leaves, the response of the various genes to nitrate is regulated transcriptionally and that the induction does not involve the synthesis of novel protein(s)in the cytosol. The significant repression of the expression of the genes for NR, NiR, GS2, and GOGATs in response to nitrate after pretreatment with EGTA or La3+ ions suggested that Ca2+ ions are involved, at least to some extent, in the signal transduction and that the induction of nitrogen-assimilatory genes by nitrate is regulated in the same or similar manner. Furthermore, the effects of inhibitors of protein kinases and protein phosphatases on the nitrate-dependent accumulation of the transcripts of genes for NR, NiR and GS2, suggested the involvement of protein phosphorylation in the regulation of the levels of transcripts of these genes.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CALCIUM ION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENE EXPRESSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUTAMATE SYNTHASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUTAMINE SYNTHETASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NITROGEN-ASSIMILATORY GENES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ZEA MAYS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sakakibara, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kobayashi, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deji, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sugiyama, T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1nn-CNVsia56utzJu1Itie7hWgpXRJXA6/view?usp=drivesdk">https://drive.google.com/file/d/1nn-CNVsia56utzJu1Itie7hWgpXRJXA6/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12845 25.06.2025 25.06.2025 Documentos solicitados