Kinetics and mechanism of heterogeneous hydrolysis of poly[(R)-3-hydroxybutyrate]fielm by PHA depolymerases (Record no. 47066)
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| fixed length control field | 02000nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153911.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-12861 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Kinetics and mechanism of heterogeneous hydrolysis of poly[(R)-3-hydroxybutyrate]fielm by PHA depolymerases |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Int. J. Biol. Macromol., 15(6), p.361-366, 1993 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The kinetics and mechanism of enzymatic degradation on the surface of poly[(R)-3-hydroxybutyrate](PC(R)-3HB]) jilm have been studied using three types of extracellular poly(hydroxyalkanoate)(PHA)depolymerases from Alcaligenes faecalis, Pseudomonas pickettii and Comamonas testosteroni. The monomer and dimer of 3-hydroxybutyric acid were produced during the course of the enzymatic degradation of PC(R)-3HB]film, and the rate of production was determined by monitoring the increase in absorbance at 210 nm on a spectrophotometer. The rate of enzymatic degradation increased to a maximum value with the concentration of PHA depolymerase, followed by a gradual decrease. The kinetic data were accountedfor in terms of a heterogeneous enzymatic reaction, involving enzymatic degradation on the surface of PC(R)-3HB]jilm via two steps of adsorption and hydrolysis by a PHA depolymerase with binding and catalytic domains. The kinetic results suggest that the properties oj-the catalytic domains are very similar among the three PHA depolymerases, but that those of the binding domains are strongly dependent on the type of depolymerase. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENZYMATIC DEGRADATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHA DEPOLYMERASES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | POLY[(R)-3-HYDROXYBUTYRATE] |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HETEROGENEOUS HYDROLYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | KINETIC MODEL |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mukai, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yamada, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Doit, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/13VXs7PmxOF3YZjJ9xswQ2fgDIC6LDAnA/view?usp=drivesdk">https://drive.google.com/file/d/13VXs7PmxOF3YZjJ9xswQ2fgDIC6LDAnA/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-12861 | 25.06.2025 | 25.06.2025 | Documentos solicitados |