Loss of Plastidic Lysophosphatidic Acid Acyltransferase Causes Embryo-Lethality in Arabidopsis (Record no. 47111)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153912.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12909
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245 10 - TITLE STATEMENT
Title Loss of Plastidic Lysophosphatidic Acid Acyltransferase Causes Embryo-Lethality in Arabidopsis
490 0# - SERIES STATEMENT
Volume/sequential designation Plant & Cell Physiology, 45(5), p.503-510, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Phosphatidic acid is a key intermediate for chloroplast membrane lipid biosynthesis. De novo phosphatidic acid biosynthesis in plants occurs in two steps: first the acylation of the sn-1 position of glycerol-3-phosphate giving rise to lysophosphatidic acid; second, the acylation of the sn-2 position of lysophosphatidic acid to form phosphatidic acid. The second step is catalyzed by a lysophosphatidic acid acyltransferase (LPAAT). Here we describe the identification of the ATS2 gene of Arabidopsis encoding the plastidic isoform of this enzyme. Introduction of the ATS2 cDNA into E. coli JC 201, which is temperature-sensitive and carries a mutation in its LPAAT gene plsC, restored this mutant to nearly wild type growth at high temperature. A greenfluorescent protein fusion with ATS2 localized to the chloroplast. Disruption of the ATS2 gene of Arabidopsis by T-DNA insertion caused embryo lethality. The development of the embryos was arrested at the globular stage concomitant with a transient increase in ATS2 gene expression. Apparently, plastidic LPAAT is essential for embryo development in Arabidopsis during the transition from the globular to the heart stage when chloroplasts begin to form.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EMBRYO DEVELOPMENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FATTY ACID BIOSYNTHESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPID METABOLISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPID TRAFFICKING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASTID PATHWAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THYLAKOID MEMBRANE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FATTY ACID BIOSYNTHESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPID METABOLISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPID TRAFFICKING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASTID PATHWAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THYLAKOID MEMBRANE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yu, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wakao, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fan, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Benning, C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1vqRNz8TrDnRZZZWq3Y6EiWpr2Mevh4_C/view?usp=drivesdk">https://drive.google.com/file/d/1vqRNz8TrDnRZZZWq3Y6EiWpr2Mevh4_C/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12909 25.06.2025 25.06.2025 Documentos solicitados