Nicotiana tabacum protoplasts secretome can evidence relations among regulatory elements of exocytosis mechanisms (Record no. 47155)
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| 000 -LEADER | |
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| fixed length control field | 02342nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153913.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-12953 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Nicotiana tabacum protoplasts secretome can evidence relations among regulatory elements of exocytosis mechanisms |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Signaling & Behavior, 6(8), p.1140-1145, 2011 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | An alternative study involving proteome analysis of the 24 h Nicotiana tabacum protoplast culture medium was performed with the aim to confirm relations among regulatory elements of exocytotic processes. Protoplasts present many convenient features to study cellular processes during transient overexpression or suppression of specific gene's products. We performed a proteomic analysis of the culture medium fraction of protoplasts transiently expressing transgenes for 24 h to characterize the effect of various regulatory proteins dominant negative mutants. A total number of 49 spots were found reproducible in the medium. Twenty-four of these spots were identified with nano RP-HPLC-ESI-MS/MS. Only three and six spots were respectively identified as canonical and non-canonical secreted cell wall proteins. The low number of spots present in the culture medium fraction allowed us the ambitious experiment to analyze the influence of various SNAREs (SYP121, SYP122, SNAP33)and Rab (Rab11)dominant negative mutants. Missing a reasonable number of identified proteins the analyses gave rise to a similarity matrix statistically analyzed considering variation within the presence of 24 spots reproducible in presence of transient overexpression of SNAREs (SYP121 and SYP122)and Rab11 native cDNAs. The similarity confirmed the closer relation between the function of SYP122 and Rab11 as evidenced by the secRGUS based analysis. This analysis included the effect of SNAP33 DN mutant and showed that this Qb-c-SNARE influence both SYP121 and SYP122 SNARE complexes. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ul-Rehman, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rinalducci, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zolla, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dalessandro, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pietro Di Sansebastiano, G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/12sndzrGvjmPyBUnyf4Ma1FY3WxznySei/view?usp=drivesdk">https://drive.google.com/file/d/12sndzrGvjmPyBUnyf4Ma1FY3WxznySei/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-12953 | 25.06.2025 | 25.06.2025 | Documentos solicitados |