Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella (Record no. 47188)

MARC details
000 -LEADER
fixed length control field 01448nam a2200193Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153913.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-12986
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 280(1), p.166-172, 2000
520 3# - SUMMARY, ETC.
Summary, etc. Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular beacons were designed to recognize a 16-base-pair region on the amplicon. As few as 2 colony-forming unit (CFU)per PCR reaction could be detected. We also demonstrated the ability of the molecular beacons to discriminate between amplicons obtained from similar species such as Escherichia coli and Citrobacter freundii in realtime PCR assays. These assays could be carried out entirely in sealed PCR tubes, enabling fast and direct detection of Salmonella in a semiautomated format
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chen, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Martinez, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mulchandani, A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1CBNx9B8h8veSAh2it0A0kCdPZqGxqLv1/view?usp=drivesdk">https://drive.google.com/file/d/1CBNx9B8h8veSAh2it0A0kCdPZqGxqLv1/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-12986 25.06.2025 25.06.2025 Documentos solicitados