MARC details
| 000 -LEADER |
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| fixed length control field |
02713nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153914.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13031 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Elevated amino acid biosynthesis in Phytophthora infestans during appressorium formation and potato infection |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Fungal Genetics and Biology, 42(3), p.244-256, 2005 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Appressorium formation is believed to be an important event in establishing a successful interaction between the late blight pathogen, Phytophthora infestans, and its host plants potato and tomato. An understanding of molecular events occurring in appressorium development could suggest new strategies for controlling late blight. We used parallel studies of the transcriptome and proteome to identify genes and proteins that are up-regulated in germinating cysts developing appressoria. As a result, Wve distinct genes involved in amino acid biosynthesis were identiWed that show increased expression in germinating cysts with appressoria. These are a methionine synthase (Pi-met1), a ketol-acid reductoisomerase (Pi-kari1), a tryptophan synthase (Pi-trp1), an acetolactate synthase (Pi-als1), and a threonine synthase (Pi-ts1). Four of these P. infestans genes were also up-regulated, although to lower levels, during the early, biotrophic phase of the interaction in potato and all Wve were considerably up-regulated during the transition(48 hpi)to the necrotrophic phase of the interaction. Real-time RT-PCR revealed that expression of potato homologues of the amino acid biosynthesis genes increased during biotrophic and necrotrophic infection phases. Furthermore, we investigated levels of free amino acids in the pre-infection stages and found that in most cases there was a decrease in free amino acids in zoospores and germinating cysts, relative to sporangia, followed by a sharp increase in germinating cysts with appressoria. Amino acid biosynthesis would appear to be important for pathogenicity in P. infestans, providing a potential metabolic target for chemical control. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTEOMICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SSH |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
REAL-TIME RT-PCR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
OOMYCETE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LATE BLIGHT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AMINO ACID BIOSYNTHESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PATHOGENICITY |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Grenville-Briggs, L.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Avrova, A.O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bruce, C.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Williams, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Whisson, S.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Birch, P.R.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Van West, P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1AHdz5qeAdTiquVa9AbfvjT1ks6redBt1/view?usp=drivesdk">https://drive.google.com/file/d/1AHdz5qeAdTiquVa9AbfvjT1ks6redBt1/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
